SIPA1 boosts migration and proliferation, and blocks apoptosis of glioma by activating the phosphorylation of the FAK signaling pathway

Journal of Medical Biochemistry

Li Shenglan¹  Zhao Jing²  Du Yuan³  Zhou Tong⁴  Liu Jiguang⁵ 

[1] Capital Medical University, Beijing Tiantan Hospital, Department of Neuro-oncology, Cancer center, Beijing, China;First Affiliated Hospital of Jiamusi University, Clinical Laboratory, Jiamusi, China;Jiamusi University, School of Basic Medicine Science, Jiamusi, China;Jiamusi University, School of Pharmacy, Jiamusi, China;Jiamusi University, School of Stomatology, Jiamusi, China;

关键词: sipa1;    fak;    phosphorylation;    glioma;   

DOI  :  10.5937/jomb0-32903

来源: DOAJ

【 摘 要 】

Background: We aimed to analyze the regulatory effects of SIPA1 (signal-induced proliferation-associated protein 1) on glioma progression and the dominant signaling pathway. Methods: Differential level of SIPA1 in glioma and normal tissues and cells was determined. Migratory, proliferative, apoptotic and cell cycle progression changes in A172 cells with overexpression or knockdown of SIPA1 were examined. Finally, protein levels of phosphorylated FAKs in A172 cells intervened by SIPA1, and the FAK inhibitor PF562271 were detected. Results: SIPA1 was upregulated in glioma cases. Knock-down of SIPA1 reduced migratory and proliferative rates of glioma cells, increased apoptotic cell rate, and declined cell ratio in the S phase. The knockdown of SIPA1 also downregulated cell cycle proteins. In addition, SIPA1 upregulated phosphorylated FAKs in A172 cells and thus boosted malignant phenotypes of glioma. Conclusions: SIPA1 is upregulated in glioma that boosts migratory and proliferative potentials of glioma cells by activating the phosphorylation of the FAK signaling pathway.

【 授权许可】

Unknown