Cell-Type-Specific Proteomics: A Neuroscience Perspective

Proteomes

AngusC. Nairn¹  RashaunS. Wilson¹ 

[1] Yale/NIDA Neuroproteomics Center, 300 George St., New Haven, CT 06511, USA;

关键词: cell type;    neuroscience;    proteomics;    mass spectrometry;    neuron;    proximity labeling;    affinity chromatography;    neuroproteomics;    biotinylation;   

DOI  :  10.3390/proteomes6040051

来源: DOAJ

【 摘 要 】

Cell-type-specific analysis has become a major focus for many investigators in the field of neuroscience, particularly because of the large number of different cell populations found in brain tissue that play roles in a variety of developmental and behavioral disorders. However, isolation of these specific cell types can be challenging due to their nonuniformity and complex projections to different brain regions. Moreover, many analytical techniques used for protein detection and quantitation remain insensitive to the low amounts of protein extracted from specific cell populations. Despite these challenges, methods to improve proteomic yield and increase resolution continue to develop at a rapid rate. In this review, we highlight the importance of cell-type-specific proteomics in neuroscience and the technical difficulties associated. Furthermore, current progress and technological advancements in cell-type-specific proteomics research are discussed with an emphasis in neuroscience.

【 授权许可】

Unknown