SXT/R391 ICE elements encode a novel ‘trap-door’ strategy for mobile element escape

Frontiers in Microbiology

Patricia eArmshaw¹  Michael P Ryan¹  Tony J Pembroke¹ 

[1] University of Limerick;

关键词: mobile genetic elements;    integrative conjugative elements (ICEs);    genetic transfer;    UV sensitisation;    ‘Trap-Door’ escape;   

DOI  :  10.3389/fmicb.2016.00829

来源: DOAJ

【 摘 要 】

Integrative Conjugative Elements (ICEs) are a class of bacterial mobile elements that have the ability to mediate their own integration, excision and transfer from one host genome to another by a mechanism of site-specific recombination, self-circularisation and conjugative transfer. Members of the SXT/R391 ICE family of enterobacterial mobile genetic elements display an unusual UV-inducible sensitisation function which results in stress induced killing of bacterial cells harbouring the ICE. This sensitisation has been shown to be associated with a stress induced overexpression of a mobile element encoded conjugative transfer gene, orf43, a traV homolog. This results in cell lysis and release of a circular form of the ICE. Induction of this novel system may allow transfer of an ICE, enhancing its survival potential under conditions not conducive to conjugative transfer.

【 授权许可】

Unknown