Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever

Brazilian Journal of Microbiology

Gisele Cristiane Gentile Cury¹  Rafaella Fabiana Carneiro Pereira¹  Luciana Maria De Hollanda¹  Marcelo Lancellotti¹ 

[1] ,State University of Campinas Institute of Biology Department of BiochemistryCampinas SP ,Brazil

关键词: Brazilian purpuric fever;    Haemophilus influenzae biogroup aegyptius;    genetic transfer;    Neisseria meningitidis;    Haemophilus influenzae;   

DOI  :  10.1590/S1517-83822014000400040

来源: SciELO

【 摘 要 】

The Brazilian Purpuric Fever (BPF) is a systemic disease with many clinical features of meningococcal sepsis and is usually preceded by purulent conjunctivitis. The illness is caused by Haemophilus influenza biogroup aegyptius, which was associated exclusively with conjunctivitis. In this work construction of the las gene, hypothetically responsible for this virulence, were fusioned with ermAM cassette in Neisseria meningitidis virulent strains and had its DNA transfer to non BPF H. influenzae strains. The effect of the las transfer was capable to increase the cytokines TNFα and IL10 expression in Hec-1B cells line infected with these transformed mutants (in eight log scale of folding change RNA expression). This is the first molecular study involving the las transfer to search an elucidation of the pathogenic factors by horizontal intergeneric transfer from meningococci to H. influenzae.

【 授权许可】

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