Scalable 3D Printed Molds for Human Tissue Engineered Skeletal Muscle

Frontiers in Bioengineering and Biotechnology

Darren J. Player¹  Julia M. Jones¹  Vivek C. Mudera¹  Richard A. Ferguson²  Andrew J. Capel²  Luke A. Baker²  Rowan P. Rimington²  Jacob W. Fleming²  Neil R. W. Martin²  Mark P. Lewis²  Mark C. Turner³ 

[1] Institute of Orthopaedics and Musculoskeletal Sciences, RNOH, University College London, London, United Kingdom;School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, United Kingdom;University Hospitals of Leicester NHS Trust, Leicester, United Kingdom;

关键词: primary skeletal muscle;    tissue engineering;    3D printing;    skeletal muscle physiology;    bioengineering;   

DOI  :  10.3389/fbioe.2019.00020

来源: DOAJ

【 摘 要 】

Tissue engineered skeletal muscle allows investigation of the cellular and molecular mechanisms that regulate skeletal muscle pathology. The fabricated model must resemble characteristics of in vivo tissue and incorporate cost-effective and high content primary human tissue. Current models are limited by low throughput due to the complexities associated with recruiting tissue donors, donor specific variations, as well as cellular senescence associated with passaging. This research presents a method using fused deposition modeling (FDM) and laser sintering (LS) 3D printing to generate reproducible and scalable tissue engineered primary human muscle, possessing aligned mature myotubes reminiscent of in vivo tissue. Many existing models are bespoke causing variability when translated between laboratories. To this end, a scalable model has been developed (25–500 μL construct volumes) allowing fabrication of mature primary human skeletal muscle. This research provides a strategy to overcome limited biopsy cell numbers, enabling high throughput screening of functional human tissue.

【 授权许可】

Unknown