Screening of polyketide genes from Brazilian Atlantic Forest soil

Universitas Scientiarum

Roger David Castillo Arteaga¹  Karen Massini¹  Leandro Maza-Garrido¹  Edith MAriela Burbano-Rosero¹  Simone Ichiwaki¹  Gabriel Padilla¹ 

[1] Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia Laboratório de Bioprodutos, Av. Prof. Lineu Prestes, 1374, São Paulo, SP, Brasil, CEP 05508-900;

关键词: bioactive compounds;    ketosynthases;    metagenomics;    polyketides;    Soil microorganisms;   

DOI  :  10.11144/Javeriana.SC22-1.sopg

来源: DOAJ

【 摘 要 】

Soil is a large source of microorganisms with potential to produce bioactive compounds. Since most of them cannot be cultured, metagenomics has become a useful tool in order to evaluate this potential. The aim of this study was to screen biosynthetic polyketide genes (PKS) present in a metagenomic library constructed from a soil sample isolated from the Brazilian Atlantic Forest. The library comprises 5000 clones with DNA inserts between 40 and 50 Kb. The characterization of the biosynthetic gene clusters of these molecules is a promising alternative to elucidate the biotechnological potential of bioactive compounds in microbial communities. The PKS genes were screened using degenerated primers. The positive clones for PKS systems were isolated, and their nucleotide sequences analysed with bioinformatics tools. The screening yielded two positive clones for PKS II genes. Furthermore, variations in the sequences of the PKS II genes from the metagenomic library were observed when compared with sequences of ketosynthases’ databases. With these findings we gain insight into the possible relation between new biosynthetic genes and the production of new secondary metabolites.

【 授权许可】

Unknown