The New Antimicrobial Peptide SpHyastatin from the Mud Crab Scylla paramamosain with Multiple Antimicrobial Mechanisms and High Effect on Bacterial Infection

Frontiers in Microbiology

Hui Peng²  Kejian Wang²  Fangyi Chen²  Kexin Zhu³  Bei Chen³  Jie Liu³  Zhongguo Shan³  Shuping Wang³  Xiaowan Ma³  Kun Qiao³ 

[1] Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources;Xiamen University;Xiamen university;

关键词: Antimicrobial peptide;    immune protection;    Membrane permeabilization;    Scylla paramamosain;    Antimicrobial mechanism;    SpHyastatin;   

DOI  :  10.3389/fmicb.2016.01140

来源: DOAJ

【 摘 要 】

SpHyastatin was first identified as a new cationic antimicrobial peptide (AMP) in hemocytes of the mud crab Scylla paramamosain. Based on the amino acid sequences deduced, it was predicted that this peptide was composed of two different functional domains, a proline-rich domain (PRD) and a cysteine-rich domain (CRD). The recombinant product of SpHyastatin displayed potent antimicrobial activities against the human pathogen Staphylococcus aureus and the aquatic animal pathogens Aeromonas hydrophila and Pseudomonas fluorescens. Compared with the CRD of SpHyastatin, the PRD presented better antimicrobial and chitin binding activities, but both regions were essential for allowing SpHyastatin complete antimicrobial activity. The binding properties of SpHyastatin to different microbial surface molecules suggested that this might be an initial and crucial step for performing its antimicrobial activities. Evaluated using propidium iodide uptake assays and scanning electron microscopy images, the antimicrobial mechanism of SpHyastatin was found to be prone to disrupt cell membrane integrity. Interestingly, SpHyastatin exerted its role specifically on the surface of S. aureus and Pichia pastoris whereas it directly killed P. fluorescens through simultaneous targeting the membrane and the cytoplasm, indicating that SpHyastatin could use different antimicrobial mechanisms to kill different species of microbes. As expected, the recombinant SpHyastatin increased the survival rate of crabs challenged with Vibrio parahaemolyticus. In addition, SpHyastatin could modulate some V. parahaemolyticus-responsive genes in S. paramamosain.

【 授权许可】

Unknown