miRNA-425-5p enhances lung cancer growth via the PTEN/PI3K/AKT signaling axis

BMC Pulmonary Medicine

Qiang Feng¹  Jiang-hao Yu¹  Jin-shan Zhou¹  Ze-shan Yang¹  Chao-Jun Huang¹  Si-yang Cheng¹ 

[1] Cardiothoracic Surgery, The Fourth Affiliated Hospital, Zhejiang University School of Medicine;

关键词: miR-425-5p;    Lung cancer;    PTEN;    PI3K/AKT signaling pathways;   

DOI  :  10.1186/s12890-020-01261-0

来源: DOAJ

【 摘 要 】

Abstract Background miRNAs regulate a multitude of cellular processes and their aberrant regulation is linked to human cancer. However, the role of miR-425-5p in lung cancer (LCa) is still largely unclear. Here, we explored the role of miR-425-5p during LCa tumorigenesis. Methods Cell proliferation was evaluated by cell counting Kit-8 and colony formation assay. Western blot and real-time PCR were accordingly used to detect the relevant proteins, miRNA and gene expression. Luciferase reporter assays were used to illustrate the interaction between miR-425-5p and PTEN. Results We demonstrate that miR-425-5p is overexpressed in LCa tissue and enhances the proliferative and colony formation capacity of the LCa cell lines A549 and NCI-H1299. Through predictive binding assays, PTEN was identified as a direct gene target and its exogenous expression inhibited the pro-cancer effects of miR-425-5p. Through its ability to down-regulate PTEN, miR-425-5p activated the PI3K/AKT axis. Conclusion We conclude that miR-425-5p promotes LCa tumorigenesis through PTEN/PI3K/AKT signaling.

【 授权许可】

Unknown