期刊论文详细信息
Current Research in Insect Science
Identification and profiling of stable microRNAs in hemolymph of young and old Locusta migratoria fifth instars
Yannick Gansemans1  Filip Van Nieuwerburgh1  Stijn Van den Brande2  Marijke Gijbels2  Dulce Santos2  Jozef Vanden Broeck2  Niels Wynant2  Paulien Peeters2 
[1] Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000, Ghent, Belgium;Research group of Molecular Developmental Physiology and Signal Transduction, KU Leuven, Zoological Institute, Naamsestraat 59 box 2465, 3000 Leuven, Belgium;
关键词: Agomir;    Ecdysteroid;    Extracellular small RNA;    Insect;    Moult;   
DOI  :  
来源: DOAJ
【 摘 要 】

Since the discovery of the first microRNA (miRNA) in the nematode Caenorhabditis elegans, numerous novel miRNAs have been identified which can regulate presumably every biological process in a wide range of metazoan species. In accordance, several insect miRNAs have been identified and functionally characterized. While regulatory RNA pathways are traditionally described at an intracellular level, studies reporting on the presence and potential role of extracellular (small) sRNAs have been emerging in the last decade, mainly in mammalian systems. Interestingly, evidence in several species indicates the functional transfer of extracellular RNAs between donor and recipient cells, illustrating RNA-based intercellular communication. In insects, however, reports on extracellular small RNAs are emerging but the number of detailed studies is still very limited. Here, we demonstrate the presence of stable sRNAs in the hemolymph of the migratory locust, Locusta migratoria. Moreover, the levels of several extracellular miRNAs (ex-miRNAs) present in locust hemolymph differed significantly between young and old fifth nymphal instars. In addition, we performed a ‘proof of principle’ experiment which suggested that extracellularly delivered miRNA molecules are capable of affecting the locusts’ development.

【 授权许可】

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