Sweet Basil Has Distinct Synthases for Eugenol Biosynthesis in Glandular Trichomes and Roots with Different Regulatory Mechanisms

International Journal of Molecular Sciences

Sarojam Rajani¹  VaishnaviAmarr Reddy¹  JessicaGambino Tjhang¹  Chunhong Li¹  Kumar Nadimuthu¹  In-Cheol Jang¹ 

[1] Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore;

关键词: eugenol synthase;    phenylpropene;    post-translational modifications;    phosphorylation;    secondary metabolism;    sweet basil;   

DOI  :  10.3390/ijms22020681

来源: DOAJ

【 摘 要 】

Production of a volatile phenylpropene; eugenol in sweet basil is mostly associated with peltate glandular trichomes (PGTs) found aerially. Currently only one eugenol synthase (EGS), ObEGS1 which belongs to PIP family is identified from sweet basil PGTs. Reports of the presence of eugenol in roots led us to analyse other EGSs in roots. We screened for all the PIP family reductase transcripts from the RNA-Seq data. In vivo functional characterization of all the genes in E. coli showed their ability to produce eugenol and were termed as ObEGS2-8. Among all, ObEGS1 displayed highest expression in PGTs and ObEGS4 in roots. Further, eugenol was produced only in the roots of soil-grown plants, but not in roots of aseptically-grown plants. Interestingly, eugenol production could be induced in roots of aseptically-grown plants under elicitation suggesting that eugenol production might occur as a result of environmental cues in roots. The presence of ObEGS4 transcript and protein in aseptically-grown plants indicated towards post-translational modifications (PTMs) of ObEGS4. Bioinformatics analysis showed possibility of phosphorylation in ObEGS4 which was further confirmed by in vitro experiment. Our study reveals the presence of multiple eugenol synthases in sweet basil and provides new insights into their diversity and tissue specific regulation.

【 授权许可】

Unknown