Mg and Ni nano-hydrotalcites modified with gold nanoparticles as platform of enzymatic electrochemical sensors for H2O2 detection

Sensing and Bio-Sensing Research

José R. Domínguez¹  Patricio Espinoza-Montero²  Gema González³  Lenys Fernández⁴  Augusto Rodríguez⁵  Omar Martínez⁶ 

[1] Corresponding author.;Instituto Venezolano de Investigaciones Científicas, Centro de Ingeniería Materiales y Nanotecnología, Caracas 89000, Venezuela;Departamento de Química, Universidad Simón Bolívar, Caracas 89000, Venezuela;Escuela de Ciencias Químicas, Pontificia Universidad Católica del Ecuador, Quito, Ecuador;Grupo CAE INIFTA (Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas), La Plata, Argentina;School of Physical Sciences and Nanotechnology, Yachay Tech University, Urcuquí 100650, Ecuador;

关键词: Golden nanoparticles;    Hydrotalcites;    Horseradish peroxidase;    Hydrogen peroxide;    Modified electrodes;   

DOI  :  

来源: DOAJ

【 摘 要 】

The immobilization of Horseradish Peroxidase enzyme over Magnesium (Mg-nHT) and Nickel (Ni-nHT) nano Hydrotalcite clays were synthesized by the co-precipitation method, and later modified with gold nanoparticles through in-situ synthesis and delamination-restructuration, is reported. The synthesized materials were characterized by Fourier-Transform Infrared Spectroscopy, X-ray diffraction, Scanning Electronic Microscopy, Flame Atomic Absorption Spectroscopy and Cyclic Voltammetry. Results show that the size of the synthetized materials is 26 nm for the gold nanoparticles, and 38.92 nm and 8.44 nm for Mg-AlnHT and Ni-AlnHT, respectively. The clays were used to modify the surface of graphite electrodes (GE); the inclusion of Au nanoparticles in the Hydrotalcites structure improved the enzyme-electrode electric communication. Figures of merit for detection of H2O2 by Differential-Pulse Voltammetry at HRP/Mg-AlnHT/GE, HRP/Ni-AlnHT/GE, HRP-AuNps/Mg-AlnHT/GE and HRP-AuNps/Ni-AlnHT/GE modified electrodes were determined, and respective detection limits of 0.012 μmolL−1, 0.014 μmolL−1, 0.009 μmolL−1, and 0.010 μmolL− 1 were obtained.

【 授权许可】

Unknown