Química Nova | |
Determinação de Cu, Fe, Mn, Zn e do teor de proteína total em amostras de trigo e soja após procedimento de extração sequencial | |
Ana Paula F. Gonçalves1  Diogo A. P. D. Lana1  Rodinei Augusti1  Adriano M. C. Pimenta1  Agenor V. dos Santos1  Leila M. Bittencourt1  Letícia M. Costa1  | |
[1] Universidade Federal de Minas Gerais; | |
关键词: sequential extraction; protein; metals; | |
DOI : 10.1590/S0100-40422012001000006 | |
来源: DOAJ |
【 摘 要 】
A sequential extraction procedure was applied to wheat and soybean seed samples. The total protein content (determined by two distinct methods: Bradford and bicinchoninic acid-BCA) and distribution of Cu, Fe, Mn and Zn in each fraction was determined. The sequential extraction employed four different solutions: water, 0.5 mol L-1 NaCl, ethanol/water (70:30 v v-1) and 0.5 mol L-1 NaOH. For both samples, the highest concentration of metals was observed in those extracts associated with globulin-type proteins using NaCl solution. Regarding protein content, higher levels were obtained using the BCA method.
【 授权许可】
Unknown