Determinação de Cu, Fe, Mn, Zn e do teor de proteína total em amostras de trigo e soja após procedimento de extração sequencial

Química Nova

Leila M. Bittencourt²  Diogo A. P. D. Lana²  Rodinei Augusti²  Letícia M. Costa²  Adriano M. C. Pimenta¹  Agenor V. Dos Santos¹  Ana Paula F. Gonçalves¹ 

[1] ,Universidade Federal de Minas Gerais Departamento de Química, Instituto de Ciências Exatas Belo Horizonte MG ,Brasil

关键词: sequential extraction;    protein;    metals;   

DOI  :  10.1590/S0100-40422012001000006

来源: SciELO

【 摘 要 】

A sequential extraction procedure was applied to wheat and soybean seed samples. The total protein content (determined by two distinct methods: Bradford and bicinchoninic acid-BCA) and distribution of Cu, Fe, Mn and Zn in each fraction was determined. The sequential extraction employed four different solutions: water, 0.5 mol L-1 NaCl, ethanol/water (70:30 v v-1) and 0.5 mol L-1 NaOH. For both samples, the highest concentration of metals was observed in those extracts associated with globulin-type proteins using NaCl solution. Regarding protein content, higher levels were obtained using the BCA method.

【 授权许可】

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