Effect of Lactobacillus johnsonii Strain SQ0048 on the TLRs-MyD88/NF-κB Signaling Pathway in Bovine Vaginal Epithelial Cells

Frontiers in Veterinary Science

Junxiang Mu¹  Chao Cheng¹  Yanru Fu²  Linchong Zhang³  Xiaohong Ma⁴  Yanming Liu⁴  Zhiguo Liu⁵  Zhenjun Li⁵  Qiaozhen Tian⁶ 

[1] College of Life Science and Technology, Jining Normal University, Jining, China;Hohhot Vocational College, Hohhot, China;Inner Mongolia Jinyu Group Co., Ltd., Hohhot, China;Inner Mongolia Shuangqi Pharmaceutical Co., Ltd., Hohhot, China;National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China;Shanxi Province Datong University, Datong, China;

关键词: Lactobacillus johnsonii;    bovine vagina epithelial cells;    TLRs-MyD88/NF- κB;    TLR2 and TLR4;    SQ0048;   

DOI  :  10.3389/fvets.2021.670949

来源: DOAJ

【 摘 要 】

Vaginal inflammation is a common disease of the dairy cows' reproductive tract. Lactic acid bacteria can combat purulent inflammation caused by pathogenic bacteria and regulate the NF-κB signaling pathway mediated by toll-like receptors (TLRs) in the inflammatory response. We studied the effect of Lactobacillus johnsonii SQ0048, an isolate with antibacterial activity, on the NF-κB signaling pathway in cow vaginal epithelial cells. The expression levels of serial effectors related to the TLRs-MyD88/NF-κB signaling pathway (TLR2, TLR4, MyD88, IKK, NF-κB, IL-1β, IL-6, TNF-α, and IL-10) were measured with real-time polymerase chain reaction (RT-PCR), ELISA, and Western blot analyses. TLR2 and TLR4 were activated by SQ0048 cells, as noted by increased mRNA expression levels of TLR2 and TLR4 in SQ0048-treated bovine vaginal epithelial cells relative to control cells (P <0.01). SQ0048 treatment also significantly increased MyD88 and IKK expression, and activated NF-κB in vaginal epithelial cells (P <0.01). In addition, SQ0048 treatment also significantly increased mRNA expression levels of IL-1β, IL-6, and TNF-α, but decreased IL-10 mRNA expression levels (P <0.01). These data indicate that strain SQ0048 presence can improve the immune functions of cow vaginal epithelial cells by activating TLRs-MyD88/NF-κB signaling pathways. However, further in vivo studies are required to confirm these findings.

【 授权许可】

Unknown