Controlling of growth performance, lipid deposits and fatty acid composition of chicken meat through a probiotic, Lactobacillus johnsonii during subclinical Clostridium perfringens infection

Lipids in Health and Disease

Research

Kangcheng Pan¹  Bo Jing²  Dong Zeng²  Lei Liu²  Jing Lai²  Xiaodan Qing²  Hesong Wang²  Xueqin Ni²  Guangyao Li³ 

[1] Animal Microecology Institute, College of Veterinary, Sichuan Agricultural University, 611130, Chengdu, China;Animal Microecology Institute, College of Veterinary, Sichuan Agricultural University, 611130, Chengdu, China;Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China;Ya’an Agricultural Science & Technology Development Co., Ltd, Ya’an, China;

关键词: Clostridium perfringens;    Lactobacillus johnsonii;    Growth performance;    Meat quality;    Lipid contents;    Fatty acid composition;   

DOI  :  10.1186/s12944-017-0408-7

 received in 2016-11-01, accepted in 2017-01-03,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMeat is considered as a major source of polyunsaturated fatty acid (PUFA) which is essential for humans, therefore its lipid level and fatty acid composition have drawn great attention. As no clinical sign can be found in chicks subclinically infected by Clostridium perfringens (CP), the meat may be purchased and eaten. The objective of the present study was to determine whether Lactobacillus johnsonii (LJ) can control the CP-caused impact on growth, lipid levels, fatty acid composition and other flavor or nutritional quality in the meat.Methods480 one-day-old chicks were divided into four groups and fed with basal diet (control and CP group). Supplemented with 1 × 105 (L-LJ) and 1 × 106 (H-LJ) colony-forming unit (cfu), CP diet was fed for 42 days. From day 19 to 22, birds of CP and LJ groups were administered with CP twice per day and the control was administered with liver broth.ResultsLJ-treated chickens were free from negative influences on growth performance and significant decrease of abdominal fat deposit., LJ inhibited CP-caused shearing force and drip loss increase and pH 40 min and 24 h decrease after sacrifice. In addition, LJ exhibited a positive effect on muscle lipid peroxidation by significantly increasing SOD, CAT and GSH-Px activity and decreasing MDA level. Besides, LJ attenuated the decrease of intramuscular fat, total cholesterol and triglyceride contents caused by CP infection. However, levels of total protein and most of amino acids were not changed. CP infection decreased C18:3n-3 (α-LA), C20:4n-6, C20:5n-3(EPA), C22:4n-6, C22:5n-3, C22:6n-3(DHA), total PUFA, n-3 PUFA and PUFA:SFA ratio and increased C14:0, total SFA and n-6:n-3 ratio. LJ was found to protect the muscle from these changes. Meanwhile, the 28-day gut permeability level was higher in CP group.ConclusionsThese findings suggest that CP may affect the growth performance of chicks and negatively influence lipid content and fatty acid composition in chicken meat. Meanwhile, LJ treatment may be effective in controlling these changes by reducing the increased gut permeability caused by CP subclinical infection.

【 授权许可】

CC BY   
© The Author(s). 2017