Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging

Photoacoustics

Terence T.W. Wong¹  Ivy H.M. Wong²  Bingxin Huang²  Atta C.Y. Chang²  Claudia T.K. Lo²  Victor T.C. Tsang²  Helen H.Y. Cheung²  Ye Tian²  Xiufeng Li²  Lei Kang²  Jack C.K. Kot² 

[1] Corresponding author.;Translational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering, Hong Kong University of Science and Technology, Hong Kong, China;

关键词: Photoacoustic microscopy;    Label-free imaging;    Tissue clearing;    Volumetric imaging;   

DOI  :  

来源: DOAJ

【 摘 要 】

Ultraviolet photoacoustic microscopy (UV-PAM) has been investigated to provide label-free and registration-free volumetric histological images for whole organs, offering new insights into complex biological organs. However, because of the high UV absorption of lipids and pigments in tissue, UV-PAM suffers from low image contrast and shallow image depth, hindering its capability for revealing various microstructures in organs. To improve the UV-PAM imaging contrast and imaging depth, here we propose to implement a state-of-the-art optical clearing technique, CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis), to wash out the lipids and pigments from tissues. Our results show that the UV-PAM imaging contrast and quality can be significantly improved after tissue clearing. With the cleared tissue, multilayers of cell nuclei can also be extracted from time-resolved PA signals. Tissue clearing-enhanced UV-PAM can provide fine details for organ imaging.

【 授权许可】

Unknown