Microgravity Affects Thyroid Cancer Cells during the TEXUS-53 Mission Stronger than Hypergravity

International Journal of Molecular Sciences

Hergen Oltmann¹  Burkhard Schmitz¹  Stefan Feldmann¹  Andreas Schütte¹  Markus Wehland²  Daniela Grimm²  Sascha Kopp²  MohamedZakaria Nassef²  Daniela Melnik²  Manfred Infanger²  ThomasJ. Bauer²  Marcus Krüger²  Herbert Schulz³  Jayashree Sahana⁴  ThomasJ. Corydon⁴  Johann Bauer⁵ 

[1] Airbus Defence and Space GmbH, Airbus-Allee 1, D-28199 Bremen, Germany;Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University Magdeburg, Leipziger Str. 44, D-39120 Magdeburg, Germany;Cologne Center for Genomics, University of Cologne, D-50931 Cologne, Germany;Department of Biomedicine, Aarhus University, Wilhelm Meyers Allé 4, DK-8000 Aarhus C, Denmark;Max Planck Institute of Biochemistry, D-82152 Martinsried, Germany;

关键词: Microgravity;    hypergravity;    random positioning machine;    thyroid cancer;    sounding rocket;    cytoskeleton;    focal adhesion;    extracellular matrix;   

DOI  :  10.3390/ijms19124001

来源: DOAJ

【 摘 要 】

Thyroid cancer is the most abundant tumor of the endocrine organs. Poorly differentiated thyroid cancer is still difficult to treat. Human cells exposed to long-term real (r-) and simulated (s-) microgravity (µg) revealed morphological alterations and changes in the expression profile of genes involved in several biological processes. The objective of this study was to examine the effects of short-term µg on poorly differentiated follicular thyroid cancer cells (FTC-133 cell line) resulting from 6 min of exposure to µg on a sounding rocket flight. As sounding rocket flights consist of several flight phases with different acceleration forces, rigorous control experiments are mandatory. Hypergravity (hyper-g) experiments were performed at 18g on a centrifuge in simulation of the rocket launch and s-µg was simulated by a random positioning machine (RPM). qPCR analyses of selected genes revealed no remarkable expression changes in controls as well as in hyper-g samples taken at the end of the first minute of launch. Using a centrifuge initiating 18g for 1 min, however, presented moderate gene expression changes, which were significant for COL1A1, VCL, CFL1, PTK2, IL6, CXCL8 and MMP14. We also identified a network of mutual interactions of the investigated genes and proteins by employing in-silico analyses. Lastly, µg-samples indicated that microgravity is a stronger regulator of gene expression than hyper-g.

【 授权许可】

Unknown