A single metabolite production by Escherichia coli BW25113 and its pflA.cra mutant cultivated under microaerobic conditions using glycerol or glucose as a carbon source

Journal of Genetic Engineering and Biotechnology

Md. Arifuzzaman¹  Kazuyuki Shimizu²  Rinty Barua³  Yasmin Akter³  Lolo Wal Marzan³  Md. Rafiqul Islam³ 

[1] Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong, Bangladesh;Department of Bioscience & Bioinformatics, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan;Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong - 4331, Bangladesh;

关键词: Glycerol;    Glucose;    Biofuel;    Escherichia coli;    pflA.cra mutant;    Ethanol production;   

DOI  :  10.1016/j.jgeb.2017.01.004

来源: DOAJ

【 摘 要 】

Abundant, low prices and a highly reduced nature make glycerol to be an ideal feedstock for the production of reduced biochemicals and biofuels. Escherichia coli has been paid much attention as the platform of microbial cell factories due to its high growth rate (giving higher metabolite production rate) and the capability of utilizing a wide range of carbon sources. However, one of the drawbacks of using E. coli as a platform is its mixed metabolite formation under anaerobic conditions. In the present study, it was shown that ethanol could be exclusively produced from glycerol by the wild type E. coli, while d-lactic acid could be exclusively produced from glucose by pflA.cra mutant, where the glucose uptake rate could be increased by this mutant as compared to the wild type strain. It was also shown that the growth rate is significantly reduced in pflA.cra mutant for the case of using glycerol as a carbon source due to redox imbalance. The metabolic regulation mechanisms behind the fermentation characteristic were clarified to some extent.

【 授权许可】

Unknown