PEMT, Δ6 desaturase, and palmitoyldocosahexaenoyl phosphatidylcholine are increased in rats during pregnancy[S]

Journal of Lipid Research

David M. Mutch¹  Flavia Badoud¹  Daniel Chalil²  Daniel M.E. Lamontagne-Kam²  Alex P. Kitson²  Jason L. Elzinga²  Kristin A. Marks²  Alan Chalil²  Juan J. Aristizabal Henao²  Ken D. Stark² 

[1] Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada;Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada;

关键词: omega-3 fatty acids;    phospholipids;    fatty acids;    nutrition/lipids;    tandem mass spectrometry;    microarray;   

DOI  :  

来源: DOAJ

【 摘 要 】

DHA is important for fetal neurodevelopment. During pregnancy, maternal plasma DHA increases, but the mechanism is not fully understood. Using rats fed a fixed-formula diet (DHA as 0.07% total energy), plasma and liver were collected for fatty acid profiling before pregnancy, at 15 and 20 days of pregnancy, and 7 days postpartum. Phosphatidylethanolamine methyltransferase (PEMT) and enzymes involved in PUFA synthesis were examined in liver. Ad hoc transcriptomic and lipidomic analyses were also performed. With pregnancy, DHA increased in liver and plasma lipids, with a large increase in plasma DHA between day 15 and day 20 that was mainly attributed to an increase in 16:0/DHA phosphatidylcholine (PC) in liver (2.6-fold) and plasma (3.9-fold). Increased protein levels of Δ6 desaturase (FADS2) and PEMT at day 20 and increased Pemt expression and PEMT activity at day 15 suggest that during pregnancy, both DHA synthesis and 16:0/DHA PC synthesis are upregulated. Transcriptomic analysis revealed minor changes in the expression of genes related to phospholipid synthesis, but little insight on DHA metabolism. Hepatic PEMT appears to be the mechanism for increased plasma 16:0/DHA PC, which is supported by increased DHA biosynthesis based on increased FADS2 protein levels.

【 授权许可】

Unknown