Reliable budding pattern classification of yeast cells with time-resolved measurement of metabolite production

BioTechniques

Kate Howell¹  Violetta Aru²  Michela Winters³  Nils Arneborg⁴ 

[1] Agricultural Science, University of Melbourne, Parkville, 3010, Australia;;Food, Faculty of Veterinary &;1School of Agriculture &2Department of Food Science, University of Copenhagen, Frederiksberg, 1958, Denmark;

关键词: budding pattern;    filamentous growth;    metabolism;    Saccharomyces cerevisiae;    yeast morphology;   

DOI  :  10.2144/btn-2021-0120

来源: DOAJ

【 摘 要 】

Filamentous growth in Saccharomyces cerevisiae is a stress response commonly induced under nutrient deprivation and by certain alcohols. It is a compound phenotype characterized by pseudohyphal growth, invasion and a shift to more polarized budding. Previous methods have not allowed the time-resolved determination of filamentous growth. Here we present a new method for budding pattern characterization that enables the measurement of filamentous growth and metabolite concentration during yeast cell growth at precise time intervals. By combining chemical cell immobilization and single-cell imaging using an oCelloScope™, this method provides more accurate budding pattern classification compared with previous methods. The applications of the method include, for example, investigation of quorum sensing-controlled yeast filamentous growth and metabolism under stress and identification of toxic metabolites.

【 授权许可】

Unknown