Characterization and Antibacterial Response of Silver Nanoparticles Biosynthesized Using an Ethanolic Extract of Coccinia indica Leaves

Crystals

Periasamy Anbu¹  Gobinath Ramachawolran²  Kishonthani Krusnamurthy²  Sumitha Samuggam²  Praveena Mariappan²  SureshV. Chinni²  Lebaka Veeranjaneya Reddy³  Shivkanya Fuloria⁴  NeerajKumar Fuloria⁴  SubashC. B. Gopinath⁵  Subramaniam Sreeramanan⁶ 

[1] Department of Biological Engineering, Inha University, Incheon 402-751, Korea;Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Kedah, Bedong 08100, Malaysia;Department of Microbiology, Yogi Vemana University, Kadapa 516005, India;Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University, Kedah, Bedong 08100, Malaysia;Institute of Nano Electronic Engineering, and School of Bioprocess Engineering, Universiti Malaysia Perlis, Kangar 01000, Perlis, Malaysia;School of Biological Sciences, University Sains Malaysia, Minden Heights, Gelugor 11800, Penang, Malaysia;

关键词: Coccinia indica;    green synthesis;    silver nanoparticle;    characterization;    antimicrobial;   

DOI  :  10.3390/cryst11020097

来源: DOAJ

【 摘 要 】

The present study was planned to characterize and analyze the antimicrobial activity of silver nanoparticles (AgNP) biosynthesized using a Coccinia indica leaf (CIL) ethanolic extract. The present study included the preparation of CIL ethanolic extract using the maceration process, which was further used for AgNP biosynthesis by silver nitrate reduction. Biosynthetic AgNPs were characterized using UV–Visible spectrometry, zeta potential analysis, transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and energy-dispersive X-ray (EDX) spectrometry. The biogenic AgNP and CIL extracts were further investigated against different bacterial strains for their antimicrobial activity. The surface plasmon resonance (SPR) signal at 425 nm confirmed AgNP formation. The SEM and TEM data revealed the spherical shape of biogenic AgNPs and size in the range of 8 to 48 nm. The EDX results verified the presence of Ag. The AgNPs displayed a zeta potential of –55.46 mV, suggesting mild AgNP stability. Compared to Gram-positive bacteria, the biogenic AgNPs demonstrated high antibacterial potential against Gram-negative bacteria. Based on the results, the current study concluded that AgNPs based on CIL extract have strong antibacterial potential, and it established that AgNP biosynthesis using CIL ethanol extract is an effective process.

【 授权许可】

Unknown