Multi Evaluation of a Modified GoldNano Carb Test for Carbapenemase Detection in Clinical Isolates of Gram-Negative Bacilli

Antibiotics

Aroonlug Lulitanond¹  Arpasiri Srisrattakarn¹  Nicha Charoensri¹  Jureerut Daduang¹  Aroonwadee Chanawong¹  Chotechana Wilailuckana¹  Jantira Pasuram²  Kunthida Khanchai³  Wichuda Phukaw⁴  Sirikan Piyapatthanakul⁵  Lumyai Wonglakorn⁶  Chutipapa Sukkasem⁶  Suthida Kenprom⁶  Waewta Kuwatjanakul⁶  Onphailin Luanphairin⁷ 

[1] Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand;Clinical Microbiology Laboratory, Buriram Hospital, Buri Ram 31000, Thailand;Clinical Microbiology Laboratory, Koh Samui Hospital, Surat Thani 84140, Thailand;Clinical Microbiology Laboratory, Lerdsin Hospital, Bangkok 10500, Thailand;Clinical Microbiology Laboratory, The Queen Sirikit National Institute of Child Health, Bangkok 10400, Thailand;Clinical Microbiology Unit, Srinagarind Hospital, Khon Kaen University, Khon Kaen 40002, Thailand;Medical Technology Laboratory, Maesot General Hospital, Tak 63110, Thailand;

关键词: Acinetobacter spp.;    AuNP;    carbapenem resistance;    Enterobacterales;    modified GoldNano Carb test;    Pseudomonas aeruginosa;   

DOI  :  10.3390/antibiotics11050684

来源: DOAJ

【 摘 要 】

Carbapenemase-producing Gram-negative bacteria have been increasingly reported. Simple and sensitive methods for carbapenemase detection are still needed. In this study, a gold nanoparticle (AuNP) solution was modified by the addition of zinc sulfate (ZnSO₄) for improving the conventional GoldNano Carb (cGoldC) test, and the modified GoldC (mGoldC) test was then evaluated for phenotypic detection of carbapenemase production in Gram-negative bacilli clinical isolates. ZnSO₄ was added to give final concentrations of 0.25, 0.5, 0.75, and 1 mM. The performance of the mGoldC test was evaluated in Enterobacterales, Acinetobacter spp., and Pseudomonas aeruginosa isolates from six hospitals in different regions using polymerase chain reaction (PCR) as a gold standard. The AuNP solution with 0.25 mM ZnSO₄ was used for the mGoldC test. Evaluation of the mGoldC test in 495 Enterobacterales, 212 Acinetobacter spp., and 125 P. aeruginosa isolates (including 444 carbapenemase producers and 388 non-carbapenemase producers) revealed sensitivity, specificity, a positive likelihood ratio, and a negative likelihood ratio of 98.6%, 98.2%, 54.7, and 0.01, respectively. This test is fast, easy to perform, cost-effective (~0.25 USD per test), and highly sensitive and specific for routine carbapenemase detection, thus leading to effective antimicrobial therapy and infection control measures.

【 授权许可】

Unknown