Standardization of unmodified gold nanoparticle (AuNPs) for detection of Actinobacillus pleuropneumoniae in swine lungs

Pesquisa Veterinária Brasileira

Maruyama, Fernanda H.¹  Pitchenin, Letícia C.²  Dutra, Valéria³  Nakazato, Luciano³  Klein, Cátia⁴  Silva, Givago F.R. da²  Chitarra, Cristiane S.²  Brandão, Laila Natasha S.² 

[1] Universidade Federal do Mato Grosso (UFMT), Cuiabá;Universidade Federal do Mato Grosso (UFMT), Cuiabá, Brasil;UFMT, Cuiabá;Embrapa Suínos e Aves, Concordia, Brasil

关键词: Swine pneumonia;    gold nanoparticle;    AuNP;    Actinobacillus pleuropneumoniae.;   

DOI  :  10.1590/S0100-736X2014000700002

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

Based on diagnostic tests for the detection of nucleic acids without amplification through the use of gold nanoparticles (AuNPs) have been described for various diseases. This study aimed to develop a technique of unmodified AuNPs to detect Actinobacillus pleuropneumoniae (App). We used 70 lung samples from pigs, 17 with and 53 without characteristic lesions of pneumonia, to detect App. The primer used was based on ApxIV gene. The AuNPs test had a sensitivity of 93.8% and specificity of 84.6% when compared with PCR detection. The results showed good agreement between AuNPs and PCR testing, and the technique can be used as an alternative to conventional tests, since it is quick and easy, and does not require implementation infrastructure and skilled labor. Index

【 授权许可】

CC BY-NC