Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq

STAR Protocols

Ahmed M. Mehdi¹  James Gotley²  Inga Rødahl³  Ian H. Frazer⁴  Meihua Yu⁴  Stacey B. Andersen⁵  Angelika N. Christ⁵  Samuel W. Lukowski⁵  Janin Chandra⁵  Emma E. Hamilton-Williams⁵ 

[1] Corresponding author;The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia;Center for Infectious Medicine, Department of Medicine, Karolinska Institute, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden;The Institute for Molecular Bioscience, The University of Queensland, Woolloongabba, QLD 4067, Australia;The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia;

关键词: Sequence analysis;    Cell isolation;    Single Cell;    Flow Cytometry/Mass Cytometry;    Health Sciences;    Sequencing;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general transferrable road map for different tissues and species.For complete details on the use and execution of this protocol, please refer to Lukowski et al. (2021).

【 授权许可】

Unknown