Lactoferrin Metal Saturation—Which Form Is the Best for Neonatal Nutrition?

Nutrients

Magdalena Strus¹  Grzegorz Majka¹  Magdalena Pilarczyk-Zurek¹  Beata Skowron²  Agnieszka Baranowska² 

[1] Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Czysta 18, 31-121 Kraków, Poland;Chair of Pathophysiology, Faculty of Medicine, Jagiellonian University Medical College, 31-121 Kraków, Poland;

关键词: lactoferrin;    bacterial translocation;    iron;    manganese;    infection;    Escherichia coli;   

DOI  :  10.3390/nu12113340

来源: DOAJ

【 摘 要 】

We evaluated the impact of metal saturation of lactoferrin (with iron and manganese) on population numbers of pathogenic species relevant for neonatal sepsis that commonly originates from the gut due to bacterial translocation. Little attention has been paid to how metal ions bound to the protein affect its activity. Several reference and clinical strains as well as probiotic strains were incubated with different forms of lactoferrin: metal-depleted (apolactoferrin), iron-saturated (hololactoferrin) and manganese-saturated lactoferrin. We also attempted to confirm the observed effects of lactoferrin forms in vivo using rat pups. The observed decrease in population numbers of Gram-negative rods could not be confirmed by quantitative plating—lactoferrin may regulate these populations diversely (e.g., by anti-biofilm activity) and contribute to the inhibition of inflammatory response. We did not see any effect of lactoferrin forms on staphylococci and bifidobacteria. However, we have noted a significant increase of population numbers of Lactobacillus strains upon incubation with manganese-saturated lactoferrin. These results were confirmed in vivo in a rat model. Metal saturation is an underestimated factor regulating lactoferrin activity. Some forms are more potent in the inhibition of pathogenic species while others, such as manganese-saturated lactoferrin, could contribute to the restoration of gut homeostasis.

【 授权许可】

Unknown