HOXD9 promote epithelial‐mesenchymal transition and metastasis in colorectal carcinoma

Cancer Medicine

Li Xiang¹  Jianjiao Lin¹  Yaying Chen²  Wenjing Zhang³  Weiyu Dai⁴  Yusi Wang⁴  Jing Wang⁴  Jiaying Li⁴  Zhizhao Lin⁴  Xiaosheng Wu⁴  Yizhi Xiao⁴  Jide Wang⁴  Mengwei Liu⁴  Ying Peng⁴  Side Liu⁴  Jing Xiong⁴  Qiong Yang⁴  Weimei Tang⁴  Linjie Hong⁴  Yang Bai⁴ 

[1] Department of Gastroenterology Longgang District Peopl Hospital Shenzhen China;Department of Gastroenterology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou China;Department of Medical Oncology The First people's Hospital of Yunnan Province Medical School of Kunming University of Science and Technology Kunming China;Guangdong Provincial Key Laboratory of Gastroenterology Department of Gastroenterology Nanfang Hospital Southern Medical University Guangzhou China;

关键词: colorectal cancer;    epithelial‐mesenchymal transition;    HOXD9;    metastasis;    TGF‐β1;   

DOI  :  10.1002/cam4.2967

来源: DOAJ

【 摘 要 】

Abstract Background HOXD9, a Hox family member, is involved in cancer growth and metastasis. But, its regulation mechanism at the molecular level particularly in colorectal cancer (CRC), is mostly unknown. Methods The HOXD9 protein expression levels were analyzed using immunofluorescence, immunohistochemistry (IHC) assays, and western blot. The in vivo and in vitro roles of HOXD9 in CRC were determined using colony formation and EdU incorporation, CCK‐8, wound scratch and transwell invasion assay, and animal models. Results Expression of HOXD9 was higher in CRC than in matched healthy tissues. High expression of HOXD9 has significantly associated with the American Joint Committee on Cancer (AJCC) stages, tumor differentiation, lymph node metastasis, and other serious invasions, and it had a poor prognosis. In vitro, HOXD9 encouraged proliferation, movement and EMT processes in cells of CRC. Also, TGF‐β1 promoted the expression of HOXD9 and this effect was dependent on the dose and downregulation of HOXD9 repressed TGF‐β1 ‐induced EMT. In vivo, HOXD9 promoted the invasive and metastasis of CRC cells via orthotopic implantation. Conclusions The ectopic expression of HOXD9 promoted the invasion metastasis in cells of the colorectal tumor by induction of EMT in vitro and vivo.

【 授权许可】

Unknown