Prevalence of virulence genes and antibiotic susceptibility of Bacillus used in commercial aquaculture probiotics in China

Aquaculture Reports

Yishan Lu¹  Kwaku Amoah²  Iddrisu Seidu³  Felix K.A. Kuebutornye⁴  Yuan Li⁵  Melody Abena Anokyewaa⁶  Berchie Asiedu⁶ 

[1] Department of Fisheries and Water Resources, University of Energy &Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Animals, Zhanjiang, 524088, China;Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China;Natural Resources, Sunyani, Ghana;Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, Guangdong, China;College of Fisheries, Guangdong Ocean University, Huguang Yan East, Zhanjiang, 524088, Guangdong, China;

关键词: Commercial probiotics;    Bacillus;    Safety;    Virulence genes;    Enterotoxin;    Antibiotic resistance;   

DOI  :  

来源: DOAJ

【 摘 要 】

Disease is a significant constraint faced by aquaculture, and its prevention and control bring together a hub of recent research. Several resources and resorts have been applied to prevent diseases in aquaculture. Probiotics are known to be beneficial natural derivatives that have several benefits in aquaculture. Currently, several commercial probiotics are used in the aquaculture industry that contains one or more live microorganisms. In the aquaculture industry, Bacillus species is one of the most widely used probiotic organisms. They are considered distinctive and are found to be natural members of the gut microbiota of some fish species. The safety of beneficial microorganisms is essential since some of these organisms are reported to harbor traits that might be transferable to their hosts. In this study, the safety of some Bacillus-based commercial probiotics used in aquaculture in terms of virulence and drug resistance were assessed. Commercial Bacillus species after isolation were screened for the presence of virulence genes (nheA, nheB, nheC, hblA, hblC, hblD, cytK, and entFM) and one emetic gene (ces), as well as their resistance to some antibiotics. Most isolates did not possess any of the virulence genes assessed. Nonetheless, three isolates harbored the nheABC and entFM enterotoxin genes, while two had the hblA, hblC, hblD, cytK genes. None of the isolates possessed the ces emetic gene. Antibiotic resistance assessment revealed most of the isolates to be resistant to β-lactam antibiotics, including penicillin, ampicillin, oxacillin, cefuroxime, and ceftriaxone, and also to minocycline.

【 授权许可】

Unknown