Protein hydrolysates from tuna cooking juice inhibit cell growth and induce apoptosis of human breast cancer cell line MCF-7

Journal of Functional Foods

Yu-Hsuan Yang¹  Kuo-Chiang Hsu²  Chuan-Chuan Hung²  Pei-Feng Kuo² 

[1] Department of Health and Nutrition Biotechnology, Asia University, No. 500, Lioufeng Road, Taichung 41354, Taiwan;Department of Nutrition, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan;

关键词: Tuna cooking juice;    Peptides;    Breast cancer;    Antiproliferation;    Cell cycle;    Apoptosis;   

DOI  :  

来源: DOAJ

【 摘 要 】

The effects of tuna cooking juice hydrolysates by protease XXIII (PA) on cell growth and induction of apoptosis of MCF-7 cell line were determined. The PA hydrolysates showed antiproliferative activities up to 25% against MCF-7 cells, and the >2.5 kDa ultrafiltration fraction (PAH2.5) possessed the highest antiproliferative activity with an IC50 value of 1.39 mg/mL. PAH2.5 induced cell cycle arrest in S phase through the increases of p21 and p27, and decrease of cyclin A expression. Further, PAH2.5 also induced apoptosis of MCF-7 cells by downregulation of the expression of Bcl-2, PARP and caspase 9, and upregulation of the expression of p53, Bax and cleaved caspase 3. Two peptides were identified in PAH2.5 as KPEGMDPPLSEPEDRRDGAAGPK (2449.292 Da) and KLPPLLLAKLLMSGKLLAEPCTGR (2562.405 Da). Thus, tuna cooking juice may be a good protein source of antiproliferative peptides against MCF-7.

【 授权许可】

Unknown