期刊论文详细信息
Cells
Fibrotic Changes and Endothelial-to-Mesenchymal Transition Promoted by VEGFR2 Antagonism Alter the Therapeutic Effects of VEGFA Pathway Blockage in a Mouse Model of Choroidal Neovascularization
YinShan Eric Ng1  Ashley Mackey1  FrancoAparecido Rossato1  Yu Su1 
[1] Department of Ophthalmology, Schepens Eye Research Institute of Mass Eye and Ear, and Harvard Medical School, 20 Staniford Street, Boston, MA 02114, USA;
关键词: endothelial-to-mesenchymal transition;    choroidal neovascularization;    age-related macular degeneration;    VEGFA;    VEGFA resistance;   
DOI  :  10.3390/cells9092057
来源: DOAJ
【 摘 要 】

Many patients with wet age-related macular degeneration do not respond well to anti- vascular endothelial growth factor A (VEGFA) therapy for choroidal neovascularization (CNV), and the efficacy of anti-VEGFA decreases over time. We investigated the hypothesis that fibrotic changes, in particular via endothelial-to-mesenchymal transition (EndoMT), play a role in CNV and alter the therapeutic effects of VEGFA pathway blockage. Induction of EndoMT of primary human retinal endothelial cells led to a significantly reduced response to VEGFA at the level of gene expression, cellular proliferation, migration, and tube formation. Suppression of EndoMT restored cell responsiveness to VEGFA. In a mouse model of spontaneous CNV, fibrotic changes and EndoMT persisted as the CNV lesions became more established over time. VEGFA receptor-2 (VEGFR2) antagonism further induced fibrosis and EndoMT in the CNV. The combination of VEGFR2 antagonism and fibrosis/EndoMT inhibition was more effective than either individual treatment in reducing CNV. Our data indicate that fibrosis and EndoMT are involved in the progression of CNV, are exacerbated by VEGFR2 inhibition, and could provide an explanation for the reduced efficacy of anti-VEGFA treatment over time.

【 授权许可】

Unknown   

  文献评价指标  
  下载次数:0次 浏览次数:0次