Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan

Parasites & Vectors

Guenter Froeschl¹  Karlygash Abdiyeva¹  Michael Hoelscher¹  Nurkeldi Turebekov¹  Stefan Frey²  Sandra Essbauer²  Josua Zinner²  Gerhard Dobler²  Lyazzat Yeraliyeva³  Ravilya Yegemberdiyeva⁴  Andrey Dmitrovsky⁴  Aksoltan Oradova⁵  Aday Amirbekov⁵  Zulfiya Kachiyeva⁵  Zhanna Shapiyeva⁶  Lyazzat Ziyadina⁶ 

[1] Center for International Health, Ludwig-Maximilians-University;Department Virology & Rickettsiology, Bundeswehr Institute of Microbiology, German Center for Infection Research;Department of Children’s Infectious Diseases, Kazakh National Medical University;Department of Infectious and Tropical Diseases, Kazakh National Medical University;Research Institute of Applied and Fundamental Medicine, Kazakh National Medical University;Scientific Practical Center of Sanitary Epidemiological Expertise and Monitoring;

关键词: Ticks;    Rickettsia slovaca;    Rickettsia raoultii;    DNA isolation;    Almaty region;    Kyzylorda region;   

DOI  :  10.1186/s13071-019-3440-9

来源: DOAJ

【 摘 要 】

Abstract Background Over 60 years ago clinical patterns resembling tick-borne rickettsioses have been described for the first time in Kazakhstan. Since 1995 the incidence of clinical cases of tick-borne rickettsioses in humans seems to be rising but studies on epidemiological data regarding the occurring etiological agents, tick vector species, prevalence and distribution throughout Kazakhstan are still scarce to date. The aim of the study was molecular investigation of ticks for spotted-fever group rickettsiae in the endemic Kyzylorda region and the so far considered as non-endemic Almaty region. A total of 2341 ticks was collected in the two regions in Kazakhstan and sorted in 501 pools: Ixodes persulcatus (243); Dermacentor marginatus (129); Haemaphysalis punctata (104); Hyalomma asiaticum (17); Dermacentor reticulatus (3); and Rhipicephalus turanicus (5). Pools were tested for Rickettsia spp. using real-time PCR. For positive samples multilocus sequence typing (MLST) was performed. Results The calculated minimum infection rate (MIR) for rickettsiae in the investigated ticks in Almaty region varied between 0.4–15.1% and 12.6–22.7% in the Kyzylorda region. At least four different Rickettsia species were identified in the two selected regions of Kazakhstan. Two of these are already known to science: Rickettsia raoultii and R. slovaca, the latter being reported for the first time in Almaty region One new form, “Candidatus R. yenbekshikazakhensis”, was described by MLST of six gene fragments in Almaty region and one new genotype, “genotype R. talgarensis” was detected using three gene fragments. Conclusions Kazakh physicians should be aware of rickettsioses after tick bites in both regions studied. Both, R. raoultii and R. slovaca should be included in the diagnostics. The role for human diseases has further to be investigated for the newly described rickettsiae, “Candidatus R. yenbekshikazakhensis” and “Genotype R. talgarensis”.

【 授权许可】

Unknown