Histological Evaluation of Periradicular Tissue Inflammatory Reactions and Calcified Tissue Formations After Implantation of Experimental Calcium Silicate and Hydroxyapatite Based Nanostructural Cements Into Root Canals of Rabbits Teeth

Acta Veterinaria

Bolka Prokić Bogomir¹  Jelena Sopta²  Slavoljub Živković³  Violeta Petrović³  Vanja Opačić-Galić³  Vukoman Jokanović⁴ 

[1] University of Belgrade, Faculty of Veterinary Medicine, Department for Surgery, Orthopedic and Ophthalmology, Serbia;University of Belgrade, Medical Faculty, Institute of Pathology, Serbia;University of Belgrade, School of Dental Medicine, Department for Restorative Dentistry and Endodontics, Belgrade, Serbia;University of Belgrade, Vinča Institute of Nuclear Sciences, Belgrade, Serbia;

关键词: biocompatibility;    calcium silicate;    hard tissue formation;    hydroxyapatite;   

DOI  :  10.2478/acve-2021-0006

来源: DOAJ

【 摘 要 】

The aim of the study was to evaluate inflammatory tissue reactions and the formation of calcified tissue after implantation of experimental nanostructured calcium silicate cement (CS) and hydroxyapatite with calcium silicate cement (HA-CS) into root canals of rabbits’ teeth. The study was conducted on four rabbits of the genus Oryctolagus cuniculus. After instrumentation and irrigation, the root canals of the central incisors were dried and filled with CS, HA-CS and control material (MTA Angelus). The animals were sacrificed after 28 days. After histological preparation and hematoxylin-eosin staining, tissue samples were evaluated for the intensity and extension of inflammatory tissue reaction; continuity, morphology and thickness of the newly formed calcified tissue; and presence of giant cells, materials particles and microorganisms. Kruskal Wallis and Dunn’s post hoc test were used for data analysis (α=0.05). There were no significant differences in the intensity of inflammatory reactions between CS, HA-CS and MTA control. HA-CS showed significantly better results than MTA and CS with respect to continuity of the newly formed calcified tissue (P=0.003 and P=0.010, respectively). Significant differences in thickness of the calcified tissue existed between CS and MTA (P=0.004) and between HA-CS and MTA (P=0.012). Application of CS and HA-CS resulted in minimal inflammatory tissue response, similar to the MTA control. CS and HA-CS were more efficient than MTA in supporting hard tissue formation. The best organized newly formed calcified tissue was seen after HA-CS application.

【 授权许可】

Unknown