ACSL4 Directs Intramuscular Adipogenesis and Fatty Acid Composition in Pigs

Animals

Haoyuan Zhang¹  Heng Wang¹  Yanzhen Bi²  Hongyan Ren²  Hongwei Xiao²  Zhe Zhu²  Liping Zhang²  Zaidong Hua²  Jiashu Tao³ 

[1] Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China;Key Laboratory of Animal Embryo Engineering and Molecular Breeding of Hubei Province, Institute of Animal Science and Veterinary Medicine, Hubei Academy of Agricultural Sciences, Wuhan 430064, China;Shandong Provincial Animal Husbandry General Station, Jinan 250022, China;

关键词: ACSL4;    intramuscular;    adipocyte;    pig;   

DOI  :  10.3390/ani12010119

来源: DOAJ

【 摘 要 】

The intramuscular fat is a major quality trait of meat, affecting sensory attributes such as flavor and texture. Several previous GWAS studies identified Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4) gene as the candidate gene to regulate intramuscular fat content in different pig populations, but the underlying molecular function of ACSL4 in adipogenesis within pig skeletal muscle is not fully investigated. In this study, we isolated porcine endogenous intramuscular adipocyte progenitors and performed ACSL4 loss- and gain-of-function experiments during adipogenic differentiation. Our data showed that ACSL4 is a positive regulator of adipogenesis in intramuscular fat cells isolated from pigs. More interestingly, the enhanced expression of ACSL4 in pig intramuscular adipocytes could increase the cellular content of monounsaturated and polyunsaturated fatty acids, such as gamma-L eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA). The above results not only confirmed the function of ACSL4 in pig intramuscular adipogenesis and meat quality attributes, but also provided new clues for the improvement of the nutritional value of pork for human health.

【 授权许可】

Unknown