Comparative transcriptomic analysis of in situ and onboard fixed deep-sea limpets reveals sample preparation-related differences

iScience

Ting Xu¹  Yi Lan¹  Pei-Yuan Qian²  Tong Wei³  Guoyong Yan⁴  Jin Sun⁴ 

[1] Department of Ocean Science, The Hong Kong University of Science and Technology, Hong Kong, China;Marine Biodiversity, Ocean University of China, Qingdao 266003, China;;Institute of Evolution &Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China;

关键词: Biotehnology;    marine biotechnology;    omics;    Transcriptomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Precise gene expression reflects the molecular response of deep-sea organisms to their harsh living environments. However, changes in environmental factors during lifting samples from the deep sea to a research vessel can also affect gene expression. By using the transcriptomic approach, we compared the gene expression profiles of the onboard fixed with the in situ fixed samples of the deep-sea limpet Bathyacmaea lactea. Our results revealed that the concomitant stress during conventional deep-sea sampling without RNA in situ fixation greatly influenced the gene expression. Various biological activities, such as cell and tissue structure, lysosomal activity, fluid balance, and unsaturated fatty acid metabolism, were perturbed, suggesting that the sampling stress has exerted systemic impacts on the life of the limpets. These findings clearly illustrate that deep-sea samples without RNA in situ fixation can easily lead to biased results in gene expression analysis, which requires to be appropriately addressed in future studies.

【 授权许可】

Unknown