The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto Mica

Applied Sciences

Vadim Yu. Tatur¹  Alexey N. Evdokimov¹  Ivan D. Shumov²  Maria O. Ershova²  Alexander I. Archakov²  Irina A. Ivanova²  Anastasia A. Valueva²  Vadim S. Ziborov²  Dmitry I. Larionov²  Tatyana O. Pleshakova²  Andrey F. Kozlov²  Yuri D. Ivanov²  Alexander Yu. Dolgoborodov³  Vladimir E. Fortov³  Alexander I. Aleshko⁴  Konstantin Yu. Sakharov⁴ 

[1] Foundation of Perspective Technologies and Novations, 115682 Moscow, Russia;Institute of Biomedical Chemistry, Pogodinskaya Str., 10, 119121 Moscow, Russia;Joint Institute for High Temperatures of the Russian Academy of Sciences, 125412 Moscow, Russia;The All-Russian Research Institute for Optical and Physical Measurements Federal State Unitary Enterprise (VNIIOFI) of the Federal Agency for Technical Regulation and Metrology of the Russian Federation, 119361 Moscow, Russia;

关键词: horseradish peroxidase;    atomic force microscopy;    protein aggregation;    shock wave;    pulsed electromagnetic field;   

DOI  :  10.3390/app112411677

来源: DOAJ

【 摘 要 】

Our present study concerns the influence of the picosecond rise-time-pulsed electromagnetic field, and the impact of nanosecond pulsed pressure on the aggregation state of horseradish peroxidase (HRP) as a model enzyme. The influence of a 640 kV/m pulsed electromagnetic field with a pulse rise-time of ~200 ps on the activity and aggregation state of an enzyme is studied by the single-molecule atomic force microscopy (AFM) method. The influence of such a field is shown to lead to aggregation of the protein and to a decrease in its enzymatic activity. Moreover, the effect of a shock wave with a pressure front rise-time of 80 ns on the increase in the HRP aggregation is demonstrated. The results obtained herein can be of use in modeling the impact of electromagnetic and pressure pulses on enzymes and on whole living organisms. Our results are also important for taking into account the effect of pulsed fields on the body in the development of drugs, therapeutic procedures, and novel highly sensitive medical diagnosticums.

【 授权许可】

Unknown