Plant Methods | |
A convenient method for simultaneous quantification of multiple phytohormones and metabolites: application in study of rice-bacterium interaction | |
关键词: Abscisic acid; defense; LC-ESI-MS; indole-3-acetic acid; jasmonic acid; phytoalexin; salicylic acid; | |
DOI : 10.1186/1746-4811-8-2 | |
来源: DOAJ |
【 摘 要 】
Abstract
Background
Simultaneous analysis of multiple functional-related phytohormones and their metabolites will improve our understanding of interactions among different hormones in the same biologic process.
Results
A method was developed for simultaneous quantification of multiple phytohormones, abscisic acid, indole-3-acetic acid (IAA), jasmonic acid (JA), and salicylic acid, hormone conjugates, IAA-aspartic acid, JA-isoleucine, and methyl JA, and phytoalexins, momilactone A, naringenin, and sakuranetin. This method combines a convenient procedure for preparing filtrated crude extracted samples and a sensitive quantification assay using ultra fast liquid chromatography-electrospray ionization tandem mass spectrometry (UFLC-ESI-MS). With this method, we determined the dynamic profiles of defense-related phytohormones, hormone metabolites, and phytoalexins in the interaction of rice with
Conclusion
This UFLC-ESI-MS method is convenient, sensitive, reliable, and inexpensive for quantification of multiple phytohormones and metabolites compared to current methods. The results obtained by application of this method in studying rice-bacterial interaction provide a basis for understanding the molecular mechanisms of rice defense responses.
【 授权许可】
Unknown