A convenient method for simultaneous quantification of multiple phytohormones and metabolites: application in study of rice-bacterium interaction

Plant Methods

Shiping Wang¹  Jinghua Xiao¹  Xianghua Li¹  Hongbo Liu¹ 

[1] National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China

关键词: salicylic acid;    phytoalexin;    jasmonic acid;    indole-3-acetic acid;    LC-ESI-MS;    defense;    Abscisic acid;   

Others  :  822708
DOI  :  10.1186/1746-4811-8-2

 received in 2011-09-28, accepted in 2012-01-15,  发布年份 2012

【 摘 要 】

Background

Simultaneous analysis of multiple functional-related phytohormones and their metabolites will improve our understanding of interactions among different hormones in the same biologic process.

Results

A method was developed for simultaneous quantification of multiple phytohormones, abscisic acid, indole-3-acetic acid (IAA), jasmonic acid (JA), and salicylic acid, hormone conjugates, IAA-aspartic acid, JA-isoleucine, and methyl JA, and phytoalexins, momilactone A, naringenin, and sakuranetin. This method combines a convenient procedure for preparing filtrated crude extracted samples and a sensitive quantification assay using ultra fast liquid chromatography-electrospray ionization tandem mass spectrometry (UFLC-ESI-MS). With this method, we determined the dynamic profiles of defense-related phytohormones, hormone metabolites, and phytoalexins in the interaction of rice with Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial blight, one of the most devastating diseases of rice worldwide.

Conclusion

This UFLC-ESI-MS method is convenient, sensitive, reliable, and inexpensive for quantification of multiple phytohormones and metabolites compared to current methods. The results obtained by application of this method in studying rice-bacterial interaction provide a basis for understanding the molecular mechanisms of rice defense responses.

【 授权许可】

   
2012 Liu et al; licensee BioMed Central Ltd.

【 图 表 】

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