A Lanosteryl Triterpene from Protorhus longifolia Improves Glucose Tolerance and Pancreatic Beta Cell Ultrastructure in Type 2 Diabetic Rats

Molecules

Phiwayinkosi V. Dludla¹  Sihle E. Mabhida²  Tryana G. Djarova²  Foluso O. Osunsanmi²  Andy R. Opoku²  Rebamang A. Mosa²  Dambudzo Penduka² 

[1] Biomedical Research and innovation Platform (BRIP), South African Medical Research Council, Tygerberg 7505, South Africa;Department of Biochemistry and Microbiology, University of Zululand, KwaDlangezwa 3886, South Africa;

关键词: type 2 diabetes;    hyperglycemia;    hyperlipidemia;    oxidative stress;    inflammation;    pancreatic beta cells;    antioxidants;    triterpenes;    Protorhus longifolia;   

DOI  :  10.3390/molecules22081252

来源: DOAJ

【 摘 要 】

Type 2 diabetes remains one of the leading causes of death worldwide. Persistent hyperglycemia within a diabetic state is implicated in the generation of oxidative stress and aggravated inflammation that is responsible for accelerated modification of pancreatic beta cell structure. Here we investigated whether a lanosteryl triterpene, methyl-3β-hydroxylanosta-9,24-dien-21-oate (RA-3), isolated from Protorhus longifolia can improve glucose tolerance and pancreatic beta cell ultrastructure by reducing oxidative stress and inflammation in high fat diet and streptozotocin-induced type 2 diabetes in rats. In addition to impaired glucose tolerance, the untreated diabetic rats showed increased fasting plasma glucose and C-peptide levels. These untreated diabetic rats further demonstrated raised cholesterol, interleukin-6 (IL-6), and lipid peroxidation levels as well as a destroyed beta cell ultrastructure. Treatment with RA-3 was as effective as metformin in improving glucose tolerance and antioxidant effect in the diabetic rats. Interestingly, RA-3 displayed a slightly more enhanced effect than metformin in reducing elevated IL-6 levels and in improving beta cell ultrastructure. Although the involved molecular mechanisms remain to be established, RA-3 demonstrates a strong potential to improve pancreatic beta cell ultrastructure by attenuating impaired glucose tolerance, reducing oxidative stress and inflammation.

【 授权许可】

Unknown