Multi-omics analyses on Kandelia obovata reveal its response to transplanting and genetic differentiation among populations

BMC Plant Biology

Yihui Zhang¹  Yuan-Ye Zhang¹  Yingjia Shen¹  Yuze Zhao¹  Pingping Liang¹  Congting Ye¹  Yifan Zhong¹  Xiaobao Pan¹ 

[1] Key Laboratory of the Ministry of E, ducation for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University;

关键词: DNA methylation;    Genetic differentiation;    Kandelia obovata;    Latitudinal gradient;    Population reciprocal transplant;    Transcriptome;   

DOI  :  10.1186/s12870-021-03123-1

来源: DOAJ

【 摘 要 】

Abstract Background Restoration through planting is the dominant strategy to conserve mangrove ecosystems. However, many of the plantations fail to survive. Site and seeding selection matters for planting. The process of afforestation, where individuals were planted in a novel environment, is essentially human-controlled transplanting events. Trying to deepen and expand the understanding of the effects of transplanting on plants, we have performed a seven-year-long reciprocal transplant experiment on Kandelia obovata along a latitudinal gradient. Results Combined phenotypic analyses and next-generation sequencing, we found phenotypic discrepancies among individuals from different populations in the common garden and genetic differentiation among populations. The central population with abundant genetic diversity and high phenotypic plasticity had a wide plantable range. But its biomass was reduced after being transferred to other latitudes. The suppressed expression of lignin biosynthesis genes revealed by RNA-seq was responsible for the biomass reduction. Moreover, using whole-genome bisulfite sequencing, we observed modification of DNA methylation in MADS-box genes that involved in the regulation of flowering time, which might contribute to the adaptation to new environments. Conclusions Taking advantage of classical ecological experiments as well as multi-omics analyses, our work observed morphology differences and genetic differentiation among different populations of K. obovata, offering scientific advice for the development of restoration strategy with long-term efficacy, also explored phenotypic, transcript, and epigenetic responses of plants to transplanting events between latitudes.

【 授权许可】

Unknown