Function verification of a chlorophyll a/b binding protein gene through a newly established tobacco rattle virus-induced gene silencing system in Kandelia obovata

Frontiers in Plant Science

Plant Science

Yunrui Shi¹  Xiaofeng Chen¹  Chonglong Wei¹  Xianfeng Wang¹  Yuhui Rao¹  Chengjin Xie²  Mingxiong Zhang³  Lu Wang⁴  Chenglang Pan⁴  Jianming Chen⁴ 

[1] Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, China;Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, China;College of Environment and Safety Engineering, Fuzhou University, Fuzhou, China;Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, China;College of Resource and Environmental Science, Fujian Agriculture and Forestry University, Fuzhou, China;Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, China;Technology Innovation Center for Monitoringand Restoration Engineering of Ecological Fragile Zonein Southeast China, Ministry of Natural Resources, Fuzhou, China;

关键词: Kandelia obovata;    VIGS system;    phytoene desaturase;    chlorophyll a/b binding protein;    carbon sequestration;   

DOI  :  10.3389/fpls.2023.1245555

 received in 2023-06-23, accepted in 2023-09-06,  发布年份 2023

来源: Frontiers

【 摘 要 】

As an important mangrove species, Kandelia obovata plays an irreplaceable role in the coastal ecosystem. However, due to a lack of genetic technology, there is limited research on its functional genes. As such, establishing an efficient and rapid functional verification system is particularly important. In this study,tobacco rattle virus (TRV) and the phytoene desaturase gene KoPDS were used as the vector and target gene, respectively, to establish a virus-induced gene silencing system (VIGS) in K. obovata. Besides, the system was also used to verify the role of a Chlorophyll a/b binding protein (Cab) gene KoCAB in leaf carbon sequestration of K. obovata.RNA-Seq and qRT-PCR showed that the highest gene-silencing efficiency could reach 90% after 10 days of inoculation and maintain above 80% after 15 days, which was achieved with resuspension buffer at pH 5.8 and Agrobacterium culture at OD600 of 0.4-0.6. Taken together, the TRV-mediated VIGS system established herein is the first genetic analysis tool for mangroves, which may greatly impel functional genomics studies in mangrove plants.

【 授权许可】

Unknown   
Copyright © 2023 Zhang, Rao, Chen, Shi, Wei, Wang, Wang, Xie, Pan and Chen