Effect of mismatch between types of viral nucleic acid and intended targets of extraction kits on polymerase chain reaction-based testing

BioTechniques

Hanjun Kim¹  Kyoung-Jin Yoon²  Jin-Ho Kim² 

[1] Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50010, USA;;1Department of Veterinary Diagnostic &

关键词: bovine herpesvirus type 1;    nucleic acid extraction kit;    polymerase chain reaction;    porcine circovirus type 2;    porcine epidemic diarrhea virus;    porcine rotavirus type A;   

DOI  :  10.2144/btn-2022-0006

来源: DOAJ

【 摘 要 】

Lab personnel generally select an extraction kit based on the nucleic acid (NA) type of the target. This study investigated the effect of mismatch between the NA type of the target and the intended target NA of the extraction kit on the polymerase chain reaction outcome. DNA, RNA and total NA extraction kits manufactured by the same company were used to isolate NA from serial dilutions of four viruses representing different genome types. All extracts were tested for the viruses by either conventional or real-time polymerase chain reactions with and without reverse transcription. While the DNA kit specifically isolated DNA from samples, the RNA kit extracted both DNA and RNA as efficiently as the total NA kit, suggesting that RNA kits can be an economical alternative.

【 授权许可】

Unknown