DESIGN OF A POLYMERASE CHAIN REACTION ASSAY FOR THE DETECTION OF bovine Herpesvirus type 1

Revista de Salud Animal

Rodríguez, Majela¹  Avila, Mislay¹  Barrera, Maritza¹  Díaz de Arce, Heidy¹ 

[1] Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba

关键词: bovine herpesvirus type 1;    diagnosis;    PCR;   

DOI  :  

学科分类：兽医学

来源: Centro Nacional de Sanidad Agropecuaria

【 摘 要 】

The design of a Polymerase Chain Reaction (PCR) assay for the detection of bovine herpesvirus type 1 (BHV-1) was described. This one was based on the amplification of a portion of the viral tk gene, for which different concentrations of primers, magnesium chloride and different annealing temperatures were tried. In addition, the parameters of analytical performance, sensitivity and specificity were evaluated with satisfactory results. Under the optimal conditions, the amplification of the band 202 pb expected was obtained, and the utility of the test for the identification of isolates was demonstrated.

【 授权许可】

CC BY-NC