Spatial and Temporal Virus Load Dynamics of SARS-CoV-2: A Single-Center Cohort Study

Diagnostics

the Lille COVID Research Network (LICORNE)¹  Ilyes Benhalima²  Enagnon Kazali Alidjinou²  Mahdi Ouafi²  Claire Tinez²  Didier Hober²  Ilka Engelmann²  Julien Goutay³  Saad Nseir³  Morgan Caplan³  Julien Poissy³  Sébastien Preau³  Karine Faure⁴  Marie-Charlotte Chopin⁴  Cécile Yelnik⁵  Marc Lambert⁵ 

[1] ;Laboratoire de Virologie, Univ Lille, CHU Lille, ULR3610, F-59000 Lille, France;Pôle de réanimation, CNRS, UMR 8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle, Univ. Lille, CHU Lille, Inserm U1285, F-59000 Lille, France;Service de Maladies infectieuses, CHU Lille, F-59000 Lille, France;Service de Médecine Polyvalente de Post-Urgence, Univ. Lille, CHU Lille, F-59000 Lille, France;

关键词: COVID-19;    SARS-CoV-2;    viral shedding;    molecular diagnostics;    RT-PCR;   

DOI  :  10.3390/diagnostics11030427

来源: DOAJ

【 摘 要 】

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an ongoing pandemic. Reverse transcription polymerase chain reaction (RT-PCR) is the gold standard for the detection of SARS-CoV-2 and has been applied to different specimen types. Understanding the virus load and virus detection frequency in different specimen types is important to improve diagnosis and estimate the duration of potential infectivity. We conducted a retrospective single-center cohort study on hospitalized and outpatients with SARS-CoV-2 infection. We analyzed the frequency of virus detection, virus load, and duration of the virus excretion in upper and lower respiratory specimens as well as stool and plasma. We found that the frequency of SARS-CoV-2 detection, the virus load, and duration of virus excretion was higher in lower respiratory tract (LRT) than in upper respiratory tract (URT) specimens. The duration of virus excretion was longer in patients requiring intensive care unit (ICU) admission. In conclusion, LRT specimens are the most appropriate specimen type for the detection and follow-up of SARS-CoV-2 infection. Duration of virus excretion is longer in severe cases of SARS-CoV-2 infection.

【 授权许可】

Unknown