Antibacterial properties of biologically formed chitosan nanoparticles using aqueous leaf extract of Ocimum basilicum

Nanomedicine Journal

Hosein Honari¹  Maryam Ghannadnia²  Iman Rasaee² 

[1] Center of Biosciences Research, Imam Hossein comprehensive University, Tehran, Iran;Department of Biotechnology, Imam Khomeini International University, Qazvin, Iran;

关键词: Biosynthesis;    Chitosan nanoparticles;    Leaf extract;    Ocimum basilicum;   

DOI  :  10.22038/nmj.2016.7580

来源: DOAJ

【 摘 要 】

Objective(s): Chitosan nanoparticles (CNPs) were prepared based on the ionic gelation of chitoan with anionic compounds of Ocimum basilicum leaf extract. Materials and Methods: After addition of Ocimum basilicum leaf extract to chitosan solution, the physicochemical properties of the nanoparticles were determined by Field Emission Scanning Electron microscope (FESEM), Fourier Transform Infrared (FTIR) analysis, X-ray diffraction (XRD) Pattern, and Dynamic Light Scattering (DLS). The antibacterial activity of CNPs was evaluated by agar disc diffusion method. Results: The synthesized nanoparticles were found to be nearly spherical shape with size in the range of 135-729 nm. FTIR analysis revealed the presence of polyphenolic; proteins and alkaloids compounds act as effective agents for converting chitosan to CNPs. Moreover, the synthesized nanoparticles showed potent antibacterial activity against Gram positive and Gram negative bacteria. Conclusion: These results reveal that natural sources of materials such as plants could be used for preparation of CNPs instead of use of chemical substances.

【 授权许可】

Unknown