NADPH-generating systems in bacteria and archaea

Frontiers in Microbiology

John eVan Der Oost¹  Servé W.M. Kengen¹  Sebastiaan K. Spaans¹  Ruud A. Weusthuis¹ 

[1] Wageningen University;

关键词: Glucose Dehydrogenases;    Hydrogenase;    Isocitrate Dehydrogenase;    Pentose Phosphate Pathway;    malic enzyme;    GaPN;   

DOI  :  10.3389/fmicb.2015.00742

来源: DOAJ

【 摘 要 】

Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is an essential electron donor in all organisms. It provides the reducing power that drives numerous anabolic reactions, including those responsible for the biosynthesis of all major cell components and many products in biotechnology. The efficient synthesis of many of these products, however, is limited by the rate of NADPH regeneration. Hence, a thorough understanding of the reactions involved in the generation of NADPH is required to increase its turnover through rational strain improvement. Traditionally, the main engineering targets for increasing NADPH availability have included the dehydrogenase reactions of the oxidative pentose phosphate pathway and the isocitrate dehydrogenase step of the tricarboxylic acid (TCA) cycle. However, the importance of alternative NADPH-generating reactions has recently become evident. In the current review, the major canonical and non-canonical reactions involved in the production and regeneration of NADPH in prokaryotes are described, and their key enzymes are discussed. In addition, an overview of how different enzymes have been applied to increase NADPH availability and thereby enhance productivity is provided.

【 授权许可】

Unknown