Neisseria meningitidis rifampicin resistant strains: analysis of protein differentially expressed

BMC Microbiology

Research Article

Maria Eugenia Schinin๠ Giuseppina Mignogna¹  Alessandra Giorgi¹  Arianna Neri²  Paola Stefanelli²  Cecilia Fazio² 

[1] Department of Biochemical Sciences, "A. Rossi Fanelli", University "Sapienza", Piazzale Aldo Moro 5, 00185, Rome, Italy;Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy;

关键词: Rifampicin;    Isocitrate Dehydrogenase;    Neisseria Meningitidis;    rpoB Gene;    Rifampicin Resistance;   

DOI  :  10.1186/1471-2180-10-246

 received in 2010-04-30, accepted in 2010-09-24,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundSeveral mutations have been described as responsible for rifampicin resistance in Neisseria meningitidis. However, the intriguing question on why these strains are so rare remains open. The aim of this study was to investigate the protein content and to identify differential expression in specific proteins in two rifampicin resistant and one susceptible meningococci using two-dimensional electrophoresis (2-DE) combined with mass spectrometry.ResultsIn our experimental conditions, able to resolve soluble proteins with an isoelectric point between 4 and 7, twenty-three proteins have been found differentially expressed in the two resistant strains compared to the susceptible. Some of them, involved in the main metabolic pathways, showed an increased expression, mainly in the catabolism of pyruvate and in the tricarboxylic acid cycle. A decreased expression of proteins belonging to gene regulation and to those involved in the folding of polypeptides has also been observed. 2-DE analysis showed the presence of four proteins displaying a shift in their isoelectric point in both resistant strains, confirmed by the presence of amino acid changes in the sequence analysis, absent in the susceptible.ConclusionsThe analysis of differentially expressed proteins suggests that an intricate series of events occurs in N. meningitidis rifampicin resistant strains and the results here reported may be considered a starting point in understanding their decreased invasion capacity. In fact, they support the hypothesis that the presence of more than one protein differentially expressed, having a role in the metabolism of the meningococcus, influences its ability to infect and to spread in the population. Different reports have described and discussed how a drug resistant pathogen shows a high biological cost for survival and that may also explain why, for some pathogens, the rate of resistant organisms is relatively low considering the widespread use of a particular drug. This seems the case of rifampicin resistant meningococci.

【 授权许可】

Unknown   
© Neri et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.