Transcriptional Profiles of Long Non-coding RNA and mRNA in Sheep Mammary Gland During Lactation Period

Frontiers in Genetics

Xinjun Zhang¹  Ling Chen²  Weihao Chen³  Xiaoyang Lv³  Shanhe Wang³  Yue Wang³  Zahid Hussain³  Wei Sun⁴  Rui Su⁶ 

[1] Animal Hospital of Yangzhou University, Yangzhou University, Yangzhou, China;Animal Science and Veterinary Medicine Bureau of Suzhou City, Suzhou, China;College of Animal Science and Technology, Yangzhou University, Yangzhou, China;College of Veterinary Medicine, Yangzhou University, Yangzhou, China;Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, China;Suzhou Taihu Dongshang Sheep Industry Development Co., Ltd., Suzhou, China;

关键词: sheep;    lactation;    lncRNA;    mRNA;    RNA-Seq;   

DOI  :  10.3389/fgene.2020.00946

来源: DOAJ

【 摘 要 】

Sheep milk and related products have been growing in popularity around the world in recent years. However, the sheep milk industry is limited by low milk yield, and the molecular regulators of ovine lactation remain largely unknown. To investigate the transcriptomic basis of sheep lactation, RNA-Sequencing was used to explore the expression profiles of lncRNA and mRNA of the mammary gland in Hu sheep at three key time points during the lactation stage: 5 days before the expected date of parturition perinatal period (PP), 6 days after parturition early lactation (EL), and 25 days after parturition peak lactation (PL). A total of 1111, 688, and 54 differentially expressed (DE) lncRNAs as well as 1360, 660, and 17 DE mRNAs were detected in the EL vs PP, PL vs PP, and PL vs EL comparisons, respectively. Several prominent mRNAs (e.g., CSN1S1, CSN1S2, PAEP, CSN2, CSN3, and COL3A1) and lncRNAs (e.g., LNC_018483, LNC_005678, LNC_012936, and LNC_004856) were identified. Functional enrichment analysis revealed that several DE mRNAs and target genes of DE lncRNAs were involved in lactation-related pathways, such as MAPK, PPAR, and ECM–receptor interaction. This study enhances our understanding of how transcriptomic profiles change during the lactation period and pave the way for future studies examining sheep lactation.

【 授权许可】

Unknown