Characterization of the Novel Phage vB_VpaP_FE11 and Its Potential Role in Controlling Vibrio parahaemolyticus Biofilms

Viruses

Qiaolan Huang¹  Juan Wang²  Yu Ding³  Hanfang Chen⁴  Zhuanbei Xie⁴  Zekun Liu⁴  Jumei Zhang⁴  Moutong Chen⁴  Meiyan Yang⁴  Qingping Wu⁴  Liang Xue⁴ 

[1] College of Agriculture, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China;College of Food Science, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China;Department of Food Science & Technology, Institute of Food Safety and Nutrition, Jinan University, Guangzhou 510642, China;Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China;

关键词: Vibrio parahaemolyticus;    aquaculture;    biofilm;    phage;    biological control;   

DOI  :  10.3390/v14020264

来源: DOAJ

【 摘 要 】

Vibrio parahaemolyticus causes aquatic vibriosis. Its biofilm protects it from antibiotics; therefore, a new different method is needed to control V. parahaemolyticus for food safety. Phage therapy represents an alternative strategy to control biofilms. In this study, the lytic Vibrio phage vB_VpaP_FE11 (FE11) was isolated from the sewers of Guangzhou Huangsha Aquatic Market. Electron microscopy analysis revealed that FE11 has a typical podovirus morphology. Its optimal stability temperature and pH range were found to be 20–50 °C and 5–10 °C, respectively. It was completely inactivated following ultraviolet irradiation for 20 min. Its latent period is 10 min and burst size is 37 plaque forming units/cell. Its double-stranded DNA genome is 43,397 bp long, with a G + C content of 49.24% and 50 predicted protein-coding genes. As a lytic phage, FE11 not only prevented the formation of biofilms but also could destroy the formed biofilms effectively. Overall, phage vB_VpaP_FE11 is a potential biological control agent against V. parahaemolyticus and the biofilm it produces.

【 授权许可】

Unknown