Lysogenic Conversion and Phage Resistance Development in Phage Exposed Escherichia coli Biofilms

Viruses

Pieter Moons¹  David Faster² 

[1] Laboratory of Food Microbiology, Department of Microbial and Molecular Systems (M²S), Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, 3001 Leuven, Belgium;

关键词: Escherichia coli;    biofilm;    lysogenic conversion;    resistance development;    Shiga toxin;    phage;   

DOI  :  10.3390/v5010150

来源: mdpi

【 摘 要 】

In this study,three-day old mature biofilms of Escherichia coli were exposed once to either a temperate Shiga-toxin encoding phage (H-19B) or an obligatory lytic phage (T7), after which further dynamics in the biofilm were monitored. As such, it was found that a single dose of H-19B could rapidly lead to a near complete lysogenization of the biofilm, with a subsequent continuous release of infectious H-19B particles. On the other hand, a single dose of T7 rapidly led to resistance development in the biofilm population. Together, our data indicates a profound impact of phages on the dynamics within structured bacterial populations.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.