Genome-Wide Characterization and Expression Analysis of NHX Gene Family under Salinity Stress in Gossypium barbadense and Its Comparison with Gossypium hirsutum

Genes

Chengzhen Liang¹  Muhammad Askari¹  Rui Zhang¹  Umar Akram¹  Sandui Guo¹  Zhigang Meng¹  Mubashir Abbas¹  Yuhan Song¹  MuhammadAli Abid¹  AyeAye Myat¹  Waqas Malik²  Zulfiqar Ali³ 

[1] Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;Genomics Lab, Department of Plant Breeding and Genetics, Faculty of Agricultural Science & Technology, Bahauddin Zakariya University, Multan 60000, Pakistan;Institute of Plant Breeding and Biotechnology, MNS-University of Agriculture, Multan 60000, Pakistan;

关键词: G. barbadense;    GbNHX;    phylogenetic;    abiotic stress;    Na⁺/H⁺ antiporter;    amiloride;   

DOI  :  10.3390/genes11070803

来源: DOAJ

【 摘 要 】

Cotton is an important economic crop affected by different abiotic stresses at different developmental stages. Salinity limits the growth and productivity of crops worldwide. Na⁺/H⁺ antiporters play a key role during the plant development and in its tolerance to salt stress. The aim of the present study was a genome-wide characterization and expression pattern analysis under the salinity stress of the sodium-proton antiporter (NHX) of Gossypium barbadense in comparison with Gossypium hirsutum. In G. barbadense, 25 NHX genes were identified on the basis of the Na⁺_H⁺ exchanger domain. All except one of the G. barbadense NHX transporters have an Amiloride motif that is a known inhibitor of Na⁺ ions in plants. A phylogenetic analysis inferred three classes of GbNHX genes—viz., Vac (GbNHX1, 2 and 4), Endo (GbNHX6), and PM (GbNHX7). A high number of the stress-related cis-acting elements observed in promoters show their role in tolerance against abiotic stresses. The Ka/Ks values show that the majority of GbNHX genes are subjected to strong purifying selection under the course of evolution. To study the functional divergence of G. barbadense NHX transporters, the real-time gene expression was analyzed under salt stress in the root, stem, and leaf tissues. In G. barbadense, the expression was higher in the stem, while in G. hirsutum the leaf and root showed a high expression. Moreover, our results revealed that NHX2 homologues in both species have a high expression under salinity stress at higher time intervals, followed by NHX7. The protein-protein prediction study revealed that GbNHX7 is involved in the CBL-CIPK protein interaction pathway. Our study also provided valuable information explaining the molecular mechanism of Na⁺ transport for the further functional study of Gossypium NHX genes.

【 授权许可】

Unknown