Characterization of the hrpZ gene from Pseudomonas syringae pv. maculicolaM2

Brazilian Journal of Microbiology

César Álvarez-mejía¹  Dalia Rodríguez-ríos¹  Gustavo Hernández-guzmán¹  Varinia López-ramírez¹  Humberto Valenzuela-soto¹  Rodolfo Marsch¹ 

关键词: hrpZ;    mutant non-pathogenic;    transmid;    Tn5;    phylogenetic;   

DOI  :  10.1590/S1517-838246320140655

来源: SciELO

【 摘 要 】

Pseudomonas syringae pv. maculicola is a natural pathogen of members of the Brassicaceae plant family. Using a transposon-based mutagenesis strategy in Pseudomonas syringaepv. maculicola M2 (PsmM2), we conducted a genetic screen to identify mutants that were capable of growing in M9 medium supplemented with a crude extract from the leaves of Arabidopsis thaliana. A mutant containing a transposon insertion in the hrpZ gene (PsmMut8) was unable to infect adult plants from Arabidopsis thaliana or Brassica oleracea, suggesting a loss of pathogenicity. The promotorless cat reporter present in the gene trap was expressed if PsmMut8 was grown in minimal medium (M9) supplemented with the leaf extract but not if grown in normal rich medium (KB). We conducted phylogenetic analysis using hrpAZB genes, showing the classical 5-clade distribution, and nucleotide diversity analysis, showing the putative position for selective pressure in this operon. Our results indicate that the hrpAZB operon from Pseudomonas syringaepv. maculicola M2 is necessary for its pathogenicity and that its diversity would be under host-mediated diversifying selection.

【 授权许可】

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